| Company Name | Street Address | Year Established | # Employees | Product/Service | Country |
| Chesapeake General Hospital | 736 North Battlefield Blvd. | 1976 | 2055 | Hospital | |
| QVC, Chesapeake, Inc. | 1553 River Birch Run North | 1988 | 1276 | Phone center/order processing facility | |
| Household Credit Services | 1421 Kristina Way | 1989 | 1215 | Regional processing center for financial services | |
| MCI/GC Services | 520 Independence Prkwy. | 1999 | 1100 | Telecommunications network operator center; directory assistance. | |
| Lifetouch National School Studios, Inc. | 2210 Paramont Avenue | 1936 | 725 | School photography processing plant | |
| Towers Perrin Administration Solutions | 1434 Crossways Blvd. | 1999 | 600 | National employee benefits administration center | |
| Reliance Staffing Services | 1215 Volvo Parkway, Suite 203 | 1982 | 600 | Employment supply service | |
| LTD Management Company, Inc. | 1564 Crossways Blvd. | 1983 | 590 | Hotel development and management | |
| Mitsubishi Chemical America, Inc. | 401 Volvo Prkwy. | 1988 | 540 | Manufacturing of organic photoconductors, aluminum composite building material and toners | Japan |
| Anteon Corporation | 1545 Crossways Blvd., Suite A | 1983 | 521 | Consulting engineers - technical services; light industrial | |
| Map Mobile | 840 Greenbrier Circle, Suite 202 | 1991 | 500 | Mobile paging service | |
| Canon Information Technology Services, Inc. | 850 Greenbrier Circle | 1995 | 500 | Technical and consumer support for all Canon manufactured products | Japan |
| Cox Communications Customer Service Center | Eden Way | 2000 | 500 | Telecommunication Services. Cable, high speed internet, digital TV and telephone | |
| Cox Communications | 815 Live Oak Drive | 1997 | 500 | Telecommunication Services. Cable, high speed internet, digital TV and telephone | |
| Sentara Home Care Services | 535 Independence Prkwy., Suite 200 | 1999 | 450 | Home care nursing, hospice, respiratory therapy, durable medical equipment, phamacy, nutritional, private duty, speech therapy, occupational therapy, physical therapy | |
| Dollar Tree Stores, Inc. | 500 Volvo Prkwy. | 1997 | 430 | Corporate headquarters and distribution facility for discount retailer | |
| Givens Trucking Co., Inc., Givens, Inc., Givens Logistics | 1720 South Military Hwy. | 1957 | 420 | Warehousing and trucking | |
| Sumitomo Machinery Corporation of America | 4200 Holland Blvd. | 1988 | 405 | Manufacturing of industrial gears and speed reducers | Japan |
| Lockheed Martin Systems Support & Training Services | 3601 Koppens Way | 1996 | 400 | Operations and maintenance of government systems | |
| First Data Resources | 1449 Kristina Way | 1999 | 400 | Card processing center | |
| Tecnico Corporation | 831 Industrial Avenue | 1993 | 400 | Ship repair, shipboard alterations and installations, steel fabrication, sheet metal fabrication, hyperbaric chamber construction, outfitting and repair, shipboard habitability/joiner fabrication, diver's air systems, information technology support | |
| Biocompatibles Eyecare, Inc. | 804 Greenbrier Circle | 1999 | 400 | Manufacture and distribute contact lens | United Kingdom |
| Smith and Keene Electric Services, Inc. | 833 Live Oak Drive | 1947 | 400 | Electrical, HVAC, plumbing contractor | |
| United States Coast Guard Finance Center | 1430-A Kristina Way | 1989 | 360 | Financial services and data warehousing for the US Coast Guard and Department of Transportation | |
| Chubb & Son, A Division of Federal Insurance Company | 600 Independence Parkway | 1998 | 350 | East coast headquarters, rapid response insurance claims center | |
| Dendrite International | 1309 Executive Blvd. | 2000 | 335 | Pharmaceutical software company | |
| Titan Service Corp/ACSS (Semcor) | 825 Greenbrier Circle, Suite M | 327 | Government contractor. Integrated services, includes design, instulation, test, technical assistance, logistics support and program mgmt. For millatary and commercial satelite and CUI systems. | ||
| SmartBeep | 840 Greenbrier Circle, Suite 104 | 1991 | 300 | Mobile paging service | |
| Tidewater Fibre Corporation | 1958 Diamond Hill Road | 1974 | 280 | Recycling and waste management; all types of recycling collection and waste removal services; recycling/disposal consulting; office recycling and confidential data destruction | |
| A Plus Temps | 3279 Western Branch Blvd., Suite C | 1998 | 275 | Temp services | |
| Vico Construction Corporation | 4001 South Military Hwy. | 1959 | 265 | Utility contractor-sewer, water, drainage; concrete contractor-curb, gutter, concrete structure; excavation/grading contractor; pressure/vacuum cleaning service; clearing debri, tubgrinding service and roadway construction; wetlands mitigation. | |
| Verizon | 505 Independence Parkway, 2nd Floor | 2000 | 261 | National telecommunications company (formerly Bell Atlantic) | |
| NOVA Chemicals, Inc. | 5100 Bainbridge Blvd. | 1973 | 260 | Manufacturing of polystyrene | |
| Competitive Media Reporting | 1122 Executive Blvd. | 1999 | 255 | Data collection center for strategic advertising intelligence and marketing information solutions to advertising agencies, broadcasters and publishers. | |
| Mid-Atlantic Coatings, Inc. | 3505 Business Center Drive | 1997 | 250 | Marine sandblasting & painting of government, cruise and commercial ships | |
| FlowServe | 3900 Cook Blvd. | 1993 | 250 | Manufacture hydraulic and industrial pumps | |
| Coastal Masonry | 813 Industrial Avenue | 1992 | 250 | Masonry contractor - commercial and industrial | |
| ICT Group | 737 Volvo Prkwy., Suite 100 | 1993 | 250 | Life Insurance Sales (JC Penney)/customer sales and support call center) | |
| Mid-Eastern Builders, Inc. | 4016 Holland Blvd. | 1982 | 242 | General contractor of commercial, industrial and utility construction. Developer, design-build and construction management services. | |
| LabCorp | 850 Greenbrier Circle, Suite N | 1992 | 237 | Medical lab testing | |
| Watson Electrical Construction Co. | 1129 Executive Blvd. | 1986 | 230 | Electrical contractor; heating and air conditioning contractor and plumbing | |
| Waste Management of Hampton Roads | 3016 Yadkin Road | 1988 | 220 | Refuse service | |
| American GFM Corporation | 1200 Cavalier Blvd. | 1977 | 210 | Manufacture of machine tools | Austria |
| Plasser American Corporation | 2001 Myers Road | 1970 | 210 | Manufacturer of railroad track maintenance machinery | Liechtenstein |
| StaffMax Corporation | 109 E. Gainsborough Square | 2000 | 202 | Temporary and contract staffing. Employee management systems. | |
| Panasonic Customer Call Center | 661 Independence Prkwy. | 1996 | 200 | Technical support center for Panasonic, Technics and Quasar products | Japan |
| ManTech Systems Engineering Corporation | 1403 Greenbrier Prkwy., Suite 300 | 1995 | 200 | Information technology; systems integration/migration; fleet logistics and maintenance support; command and control; combat systems support | |
| Frito Lay, Inc. | 909 Professional Place | 200 | Distribution of salty snacks | ||
| Navy & Marine Corp Uniform Support Center | 1545 Crossways Blvd., Suite 200 | 1992 | 200 | Distribution and sale of Navy and Marine Corp uniforms | |
| Lowe's of Chesapeake, Inc. | 1308 North Battlefield Blvd. | 1995 | 200 | Building materials, hardware and home improvement merchandise | |
| Raytheon Technical Services Company, ROTHR | 2 Relay Road, Building 344 | 1988 | 200 | Electronic systems and equipment; operations and maintenance services; engineering services | |
| k.van Bourgondien of Virginia, Inc. | 1920 Campostella Road | 1984 | 200 | Manufacturing of garden products; import and distribution of garden products and flowerbulbs | The Netherlands |
| Arc Electric, Inc. | 500 Woodlake Drive, Suite 105 | 1973 | 200 | Industrial and commercial electrical contractor | |
| Lockheed Martin Corporation, ASW Support Center | 500 Woodlake Drive, Suite 2 | 1996 | 200 | Antisubmarine warfare support; engineering systems support and service; logistics support and service | |
| Sprinkle Masonry Company, Inc. | 1030 Ruritan Blvd. | 1960 | 200 | Masonry subcontractor | |
| HUDD Distributors | 3025 Gum Court | 2001 | 197 | Logistics support including distribution, warehousing, packaging and transportation | |
| Chesbay Distributing Co., Inc. | 3928 Cook Blvd. | 1983 | 195 | Distribution of beers | |
| Johnson Controls | 3824 Cook Blvd. | 1995 | 187 | Manufacturing of automotive seats for Ford Trucks | |
| White's Nursery and Greenhouses | 3133 Old Mill Road | 1957 | 184 | Greenhouse, nursery and garden center | |
| Norfolk Dredging Company | 110 N. Centerville Turnpike | 1958 | 180 | Dredging services and land reclamation | |
| Med Systems Associates LP | 3241 Western Branch Blvd., Suite A | 1992 | 180 | Medical billing service; medical practice management | |
| Moore's Lumber & Building Supply | 404 Greentree Road | 1985 | 180 | Building materials including lumber and hardware | |
| AudioVox | 630-B Woodlake Drive | 1991 | 175 | Assembly, warehousing and distribution of electronic automotive accessories | |
| Kempsville Building Materials, Inc. | 3300 Business Center Drive | 1991 | 175 | Distribution of building materials and supplies; door and window assembly; wood trusses; stair shop; custom mill work | |
| TDS, Inc. | 1501 Crossways Blvd., Suite A | 1993 | 175 | Electronics research, development and services firm | |
| BAE Systems North America, Inc. | 821 Live Oak Drive | 1978 | 170 | Design, integration, assembly, installation and maintenance of communications and information security systems; unique design and fabrication of mobile electronic systems; complex cable fabrication of all sizes and materials | |
| Hampton Roads Mechanical Contractors, Inc. | 932 Corporate Lane | 1985 | 170 | Industrial piping, heating, ventilation and air conditioning & plumbing | |
| Associated Distributors, Inc. | 401 Woodlake Drive | 1987 | 170 | Wholesaler of wines, beers, distilled spirits and bar supplies | |
| Jones Transfer and Warehouse; D.D. | 2626 Indian River Road | 1954 | 160 | Logistics support including distribution, warehousing, packaging and transportation | |
| Boise Cascade Office Products | 1100 International Plaza South | 1994 | 160 | Office supplies and furniture; computers and technology | |
| Minton and Roberson, Inc. | 650 Woodlake Drive | 160 | Sheet metal fabrication | ||
| Armada/Hoffler Holding Company | 1435 Crossways Blvd. | 1987 | 151 | Wholly owned and affliated companies that provide a full range of client-driven real estate services including development, design, construction, sales, financing and asset management. | |
| Oceaneering/CLT | 804-D Industrial Avenue | 1988 | 150 | Marine repair | |
| Higgerson & Buchanan, Inc. | 5300 Bainbridge Blvd. | 1960 | 150 | Heavy construction | |
| Yupo Corporation America | 800 Yupo Court | 1996 | 150 | Manufacture synthetic paper | Japan |
| Chesapeake Hardwood Products, Inc. | 201 Dexter Street, West | 1989 | 140 | Manufacturer of prefinished interior paneling and hardwood plywood, cut-to-size furniture components, ECO No-Oil concrete form panels and Diamondized UV-finished hardwoods, distribution of imported hardwood plywood, lumber, laminate flooring | Indonesia |
| Volvo Penta of the Americas, Inc. | 1300 Volvo Penta Drive | 1983 | 135 | Marketing, engineering, production of marine engines and transmissions | Sweden |
| Heard Concrete Construction Corporation | 3527 Business Center Drive | 1989 | 135 | Concrete contractor, commercial & industrial | |
| First Data Resources Output Services Division | 1408 Stephanie Way | 2000 | 130 | Print and mail operation | |
| Electric Motor & Contracting Co., Inc. | 3703 Cook Blvd. | 1983 | 130 | Electric motor repair; electrical apparatus repair and service; machine shop | |
| ISS Business Solutions | 700 Independence Parkway, Suite 500 | 2001 | 126 | Information technology services, Including help desk lan/ wan design installation, training and mantinance. | |
| Ballance Electrical Service, Inc.; L.E. | 944 Corporate Lane | 1971 | 125 | Electrical contractor, commercial and industrial construction | |
| East Coast Abatement & Demolition Company, Inc. | 2530 Centerville Turnpike S. | 1987 | 125 | Asbestos, lead and full hazardous material cleanup and disposal transportation; full building demolition/implosion/site clearing/transportation | |
| Anchor Services, Inc. | 933 Ventures Way (1390 Progress Road, Suffolk, 23434) | 1985 | 125 | Vending services | |
| Precon Construction Company | 1401 Precon Drive, Suite 101 | 1985 | 120 | Utility construction and site development | |
| GSTEK, Inc. | 1100 Madison Plaza, Suite A | 1992 | 120 | Telecommunications systems design | |
| McLean Contracting Company | 100 Republic Road | 1940 | 120 | Industrial and marine construction; bridges, piers and bulkheads | |
| Usui International Corporation | 550 Woodlake Circle, #A | 1990 | 120 | Manufacturer of fuel lines | Japan |
| VSE Corporation | 500 Woodlake Drive, Suite 1 | 1980 | 119 | Government contracting: Naval engineering, technical support | |
| Browning-Ferris Industries | 3821 Cook Blvd. | 1984 | 115 | Waste removal, disposal and recycling services | |
| Williams, Inc.; E.V. | 120 South Dominion Blvd. | 1982 | 112 | Asphalt paving; grading and site work; Hwy. construction; asphalt sales | |
| General Dynamics Electronics Systems Division | 816 Greenbrier Circle, Suite 209 | 1992 | 112 | Government contracting, electronics services and electronics defense | |
| Curling Welding Service; W.E. | 4125 South Military Hwy. | 1975 | 110 | Gas line construction and underground utilities | |
| Damuth Services, Inc. | 1100 Cavalier Blvd. | 1997 | 110 | Comfort systems: heating, air conditioning and control systems for commercial and industrial applications. Service and maintenance for commercial systems. Parts & supplies for residential and commercial systems | |
| DaiEi Papers (USA), Inc. | 550 Woodlake Drive | 2000 | 105 | Paper processing and conversion. | Japan |
| Brink's Inc. | 1223 Executive Blvd. | 1992 | 103 | Financial collection and distribution | |
| Wilson Trucking Corporation | 1005 Enterprise Court | 2000 | 103 | Trucking company operating in mid-atlantic/southeast U.S. Common carrier handling truckloads and containers. | |
| CACI | 501 Independence Prkwy., Suite 325 | 1999 | 100 | Research center. High user-value systems and service through information sciences, systems, engineering, custom software, simulation, imaging, communications integration and proprietary database and software products. | |
| Westar Roofing Corporation | 805 Live Oak Drive | 100 | Roofing construction and installation | ||
| DaviShar Group, Inc. | 820 Greenbrier Circle, Suite 1 | 1994 | 100 | Plumbing contractor, home remodeling, renovation, service plumbing and water treatment | |
| Southern Atlantic Label & Screen Printing Company, Inc. | 1300 Cavalier Blvd. | 1972 | 100 | Custom manufacturers of identification items; screen printing; flexographic printing | |
| Tidewater Finance Company | 565 Cedar Road, Suite 1 | 1991 | 100 | Consumer installment finance company | |
| Box USA | 723 Fenway Avenue | 1963 | 100 | Corrugated boxes | |
| Colonial Downs OTW | 3376 Military Hwy. South | 1996 | 100 | Satellite wagering facility |


